Presqualene metabolism in two species of Lagenidium

• Published in: Canadian journal of microbiology Vol. 40; no. 10; p. 858
• Main Authors: Gallagher, P.A. (University of North Carolina, Chapel Hill, NC.), Warner, S.A, Domnas, A.J
• Format: Journal Article
• Language: English
• Published: Canada 01.10.1994
• Subjects: Acetates - metabolism; ACIDE GRAS; ACIDE ORGANIQUE; ACIDOS GRASOS; ACIDOS ORGANICOS; Acyl Coenzyme A - metabolism; AUXILIAIRE DE LUTTE BIOLOGIQUE; BIOLOGICAL CONTROL ORGANISMS; CHAMPIGNON ENTOMOPATHOGENE; CHOLESTEROL; COLESTEROL; ENTOMOGENOUS FUNGI; ESTER; ESTERES; ESTERS; FATTY ACIDS; Fatty Acids - biosynthesis; FUNGAL INSECTICIDES; GERANIOL; Glucose - metabolism; HONGOS ENTOMOPATOGENOS; INSECTICIDAS; INSECTICIDE; INSECTICIDES; LAGENIDIUM CALLINECTES; LAGENIDIUM GIGANTEUM; Lipid Metabolism; LIPIDE; LIPIDOS; LIPIDS; LIPOGENESE; LIPOGENESIS; METABOLISME DES STEROIDES; METABOLISMO DE ESTEROIDES; MEVALONIC ACID; Mevalonic Acid - metabolism; Oomycetes - enzymology; Oomycetes - metabolism; ORGANIC ACIDS; ORGANISMOS PARA CONTROL BIOLOGICO; STEROID METABOLISM; STEROIDOGENESIS; Sterols - biosynthesis; TRIACYLGLYCEROLS; TRIGLICERIDOS; TRIGLYCERIDE; TRIGLYCERIDES
• ISSN: 0008-4166; 1480-3275
• DOI: 10.1139/m94-136

The fate of precursors of the isoprenoid pathway was studied in the sterol auxotroph Lagenidium giganteum and in the positive control organism Lagenidium callinectes. Acetate derived from glucose and mevalonic acid was converted to sterols and fatty acids in L. callinectes. Lagenidium giganteum converted mevalonic acid to sterols and fatty acids, but glucose-derived acetate was not utilized for sterol synthesis. The results showed that the defect in the isoprenoid pathway of L. giganteum occurs at the level of the beta-hydroxy-beta-methylglutarylcoenzyme A reductase-synthase complex. Various aspects of this defect are discussed relative to metabolism of the organism.