A self-assembled gold-spiked nanosphere based paper assay for sensitive glucose detection

• Published in: Analytical methods Vol. 17; no. 19; pp. 42 - 48
• Main Authors: Dai, Xin-Yi, Shi, Xiao-Jie, Wu, Yu, Zhang, Ting-Ting, Song, Tian-Shun, Li, Xiang-Ling, Xie, Jing-Jing
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 15.05.2025
• Subjects: Biomedical materials; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Catalytic activity; Glucose; Glucose - analysis; Glucose oxidase; Glucose Oxidase - chemistry; Glucose Oxidase - metabolism; Gold; Gold - chemistry; Humans; Limit of Detection; Metal Nanoparticles - chemistry; Nanospheres; Nanospheres - chemistry; Self-assembly; Sweat; Sweat - chemistry
• ISSN: 1759-9660; 1759-9679; 1759-9679
• DOI: 10.1039/d5ay00306g

Glucose detection plays a crucial role in health monitoring, and the development of efficient and convenient detection platforms is highly desired. This study presents a sensitive and convenient detection system for non-invasive glucose detection in sweat, which is formed by combining the self-assembled gold-spiked nanosphere (GSN) nanozyme with paper-based analytical devices (PADs). The GSN nanozyme exhibits excellent glucose oxidase-like catalytic activity, significantly enhancing the sensitivity and selectivity of glucose detection. By integrating the self-assembled GSN nanozyme with a paper-based assay, this system enables fast, cost-effective, and non-invasive glucose detection and shows minimal interference from common substances found in sweat, demonstrating its potential for practical biomedical applications. We constructed a gold-spiked nanosphere nanozyme-based paper platform for non-invasive detection of glucose.