Influence of polyphenol-metal ion-coated ovalbumin/sodium alginate composite nanoparticles on the encapsulation of kaempferol/tannin acid

• Published in: International journal of biological macromolecules Vol. 209; no. Pt A; pp. 1288 - 1297
• Main Authors: Tao, Xiaoya, Shi, Hang, Cao, Ailing, Cai, Luyun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2022
• Subjects: Alginates - chemistry; Anti-Bacterial Agents - pharmacology; Antioxidants - chemistry; Antioxidants - pharmacology; Composite nanoparticles; Encapsulation; Escherichia coli; Kaempferol; Kaempferols - pharmacology; Metal Nanoparticles; Nanoparticles - chemistry; Ovalbumin; Polyphenols; Sodium alginate; TA-Fe3+ coating film; Tannic acid; Tannins; Tannic acid; TA-Fe3+ coating film; Encapsulation; Ovalbumin; Kaempferol; Composite nanoparticles; Sodium alginate; TA-Fe(3+) coating film
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.04.108

In this research, ovalbumin (OVA) and sodium alginate (SA) were used as the materials to prepare OVA-SA composite carriers, which protected and encapsulated the hydrophobic kaempferol (KAE) and the hydrophilic tannic acid (TA). To achieve the purpose of targeted delivery, the TA-Fe3+ coating film was prepared. Results showed that the observation of small diffraction peaks in carriers proved the formation of TA/Fe3+ coating film on the surface of four composite nanoparticles (pOVA, pOVA-SA, pOVA-KAE-SA, and pOVA-KAE-TA-SA). The protein structure of the composite nanoparticles coated with TA/Fe3+ changed, and the order of the changes was pOVA-KAE > pOVA > pOVA-KAE-SA > pOVA-KAE-TA-SA > pOVA-SA. This phenomenon is due to the fact that the chromophore –C=O and the auxo-chromophore –OH are in the opposite position in the benzene ring of TA, and the two substituents have opposite effects and synergize, resulting in the different degrees of redshift of the composite nanoparticle λmax. Additionally, pOVA-SA had the highest α-helix content and the lowest random coils, conferring the protein structure the strongest stability. The coating of TA/Fe3+ increased the system stability and the thermal stability of the composite nanoparticles. Additionally, the carriers were endowed with antioxidant activity, and their antibacterial ability against Staphylococcus aureus and Escherichia coli was pOVA-KAE-TA-SA > pOVA-KAE-SA > pOVA-KAE > pOVA-SA > pOVA based on the difference in antibacterial diameter (D, mm) and square (S, mm2). pOVA-KAE-TA-SA had the strongest antioxidant activity and antibacterial ability, which improved the bioavailability of TA/KAE. These results provide a theoretical basis for the application of OVA-SA composite nanoparticles in the delivery of bioactive compounds. •OVA/SA composite carrier encapsulated KAE and TA was prepared.•The TA-Fe3+ coating film with targeted release was formed.•The protein structure of composite nanoparticles coated with TA-Fe3+ changed.•The coating film increased system and thermal stability of nanocomposites.•pOVA-KAE-TA-SA had the strongest antioxidant activity and antibacterial ability.