Association of mesenchymal stem cells with platelet rich plasma on the repair of critical calvarial defects in mice

• Published in: Acta cirurgica brasileira Vol. 27; no. 3; pp. 201 - 209
• Main Authors: Monteiro, Betânia Souza, Del Carlo, Ricardo Junqueira, Argôlo-Neto, Napoleão Martins, Nardi, Nance Beyer, Carvalho, Pablo Herthel, Bonfá, Laila de Paula, Chagastelles, Pedro César, Moreira, Higo Nasser, Viloria, Marlene Isabel Vargas, Santos, Bianka Souza dos
• Format: Journal Article
• Language: English
• Published: Brazil 01.03.2012
• Subjects: Adult Stem Cells - transplantation; Adult Stem Cells - ultrastructure; Animals; Bone Marrow Cells - physiology; Bone Marrow Cells - ultrastructure; Bone Regeneration - physiology; Cells, Cultured; Disease Models, Animal; Green Fluorescent Proteins - genetics; Male; Mesenchymal Stem Cell Transplantation - methods; Mesenchymal Stromal Cells - physiology; Mesenchymal Stromal Cells - ultrastructure; Mice; Mice, Inbred C57BL; Mice, Transgenic; Osteogenesis - physiology; Platelet-Rich Plasma - physiology; Random Allocation; Skull - injuries; Skull - surgery; Skull - ultrastructure; Tissue Engineering - methods; Transplantation, Homologous
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/S0102-86502012000300001

To evaluate the effects of mesenchymal stem cells (MSC) from eight mice C57BL/6 gfp(+) bone marrows expanded in cultures associated with platelets rich plasma (PRP) deriving from another eight mice, in the repair of critical defects in calvarial bone produced in twenty-four adult isogenic mice C57BL/6. The animals were submitted to a cranial defect of 6.0mm in diameter and divided into two equal experimental groups. Control group did not receive treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL associated with 50.0 µL of plasma gel containing 1.0 x 10(9) autologous platelets within the defect. In the treated group was observed process of angiogenesis and bone repair better than control group. Mesenchymal stem cells derived from bone marrow of C57BL/6 gfp(+) mice associated with PRP gel applied in bone critical defects produced in calvarial contributes positively to the process of bone repair.