Identification of the Regulators Binding to the Upstream Region of glxR in Corynebacterium glutamicum

• Published in: Journal of microbiology and biotechnology Vol. 25; no. 8; pp. 1216 - 1226
• Main Authors: Subhadra, Bindu, Ray, Durga, Han, Jong Yun, Bae, Kwang-Hee, Lee, Jung-Kee
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 01.08.2015
• Subjects: Carbon - metabolism; Chromatography, Affinity; Corynebacterium glutamicum - genetics; DNA, Bacterial - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - isolation & purification; Electrophoretic Mobility Shift Assay; Gene Deletion; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Promoter Regions, Genetic; Protein Binding; Transcription Factors - genetics; Transcription Factors - isolation & purification; 생물학; Adenylate cyclase; GntR3; RamB; Corynebacterium glutamicum; SucR; GlxR
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1502.02053

GlxR is considered as a global transcriptional regulator controlling a large number of genes having broad physiological aspects in Corynebacterium glutamicum. However, the expression profile revealing the transcriptional control of glxR has not yet been studied in detail. DNA affinity chromatography experiments revealed the binding of transcriptional regulators SucR, RamB, GlxR, and a GntR-type protein (hereafter denoted as GntR3) to the upstream region of glxR. The binding of different regulators to the glxR promoter was confirmed by EMSA experiments. The expression of glxR was analyzed in detail under various carbon sources in the wild-type and different mutant strains. The sucR and gntR3 deletion mutants showed decreased glxR promoter activities, when compared with the wild type, irrespective of the carbon sources. The promoter activity of glxR was derepressed in the ramB deletion mutant under all the tested carbon sources. These results indicate that SucR and GntR3 are acting as activators of GlxR, while RamB plays a repressor. As expected, the expression of glxR in the cyaB and glxR deletion mutants was derepressed under different media conditions, indicating that GlxR is autoregulated.