Sciatic nerve regeneration in rats by a nerve conduit engineering with a membrane derived from natural latex

To evaluate the capacity of natural latex membrane to accelerate and improve the regeneration quality of the of rat sciatic nerves. Forty male adult Wistar rats were used, anesthetized and operated to cut the sciatic nerve and receive an autograft or a conduit made with a membrane derived from natur...

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Published inActa cirurgica brasileira Vol. 27; no. 12; pp. 885 - 891
Main Authors Ganga, Marcos Vinícius Muniz, Coutinho-Netto, Joaquim, Colli, Benedicto Oscar, Marques Junior, Wilson, Catalão, Carlos Henrique Rocha, Santana, Ricardo Torres, Oltramari, Marcos Roberto Pedron, Carraro, Kleber Tadeu, Lachat, João-José, Lopes, Luiza da Silva
Format Journal Article
LanguageEnglish
Published Brazil 01.12.2012
Subjects
Animals
Latex - therapeutic use
Male
Nerve Regeneration
Nerve Tissue - anatomy & histology
Rats
Rats, Wistar
Sciatic Nerve - physiology
Sciatic Nerve - surgery
Wound Healing - physiology
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Summary:To evaluate the capacity of natural latex membrane to accelerate and improve the regeneration quality of the of rat sciatic nerves. Forty male adult Wistar rats were used, anesthetized and operated to cut the sciatic nerve and receive an autograft or a conduit made with a membrane derived from natural latex (Hevea brasiliensis). Four or eight weeks after surgery, to investigate motor nerve recovery, we analyzed the neurological function by walking pattern (footprints analysis and computerized treadmill), electrophysiological evaluation and histological analysis of regenerated nerve (autologous nerve graft or tissue cables between the nerve stumps), and anterior tibial and gastrocnemius muscles. All functional and morphological analysis showed that the rats transplanted with latex conduit had a better neurological recovery than those operated with autologous nerve: quality of footprints, performance on treadmill (p<0.01), electrophysiological response (p<0.05), and quality of histological aspects on neural regeneration. The data reported showed behavioral and functional recovery in rats implanted with latex conduit for sciatic nerve repair, supporting a complete morphological and physiological regeneration of the nerve.
ISSN:0102-8650
1678-2674
0102-8650
DOI:10.1590/S0102-86502012001200010