An online derivatization strategy targeting carbon–carbon double bonds by laser-ablation carbon fiber ionization mass spectrometry imaging: Unraveling the spatial characteristic in mountain-cultivated ginseng and garden-cultivated ginseng with different ages

• Published in: Food chemistry Vol. 410; p. 135365
• Main Authors: Lu, Yingjie, Cao, Yuqi, Chen, Danqing, Zhou, Yaobin, Zhang, Li, Su, Yue, Guo, Yinlong
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2023
• Subjects: Carbon - metabolism; Carbon Fiber; Carbon–carbon double bonds; Garden-cultivated ginseng; Gardens; Lasers; Mass Spectrometry; Mass spectrometry imaging; Mountain-cultivated ginseng; Online derivatization; Panax - chemistry; Mountain-cultivated ginseng; Garden-cultivated ginseng; Online derivatization; Mass spectrometry imaging; Carbon–carbon double bonds
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2022.135365

[Display omitted] •Online derivatization imaging strategy were developed targeting CC in ginseng.•Isotope labelled derivatization promoted by laser ablation carbon fiber ionization.•52 bioactive metabolites were detected in mountain- and garden-cultivated ginseng.•Spatial location differences were found in two ginseng species with various ages.•Mountain-cultivated ginseng held high correlation among bioactive compounds. Serving as a world-renowned tonic, ginseng contains various types of bioactive metabolites. The comprehensive profiling of these metabolites may help explore the nutritional value of ginseng. Due to high variety in chemical structures, simultaneous monitoring of these metabolites remains a challenge. Herein, a high-throughput and high-selectivity online derivatization mass spectrometry imaging strategy targeting CC was developed. As a widely existed chemical group, CC acts like a bridge connecting different kinds of metabolites. [d0]/[d10]-Bis(pyridine) iodine tetrafluoroboride reagent was chosen for the derivatization of CC, the detection sensitivity of which increased about 3 magnitudes after derivatization. Assisted by laser ablation carbon fiber ionization mass spectrometry, the spatial distribution of bioactive metabolites in mountain-cultivated and garden-cultivated ginseng were visualized. The correlation heatmap results revealed that metabolites in mountain-cultivated ginseng hold higher correlation than those in garden-cultivated ginseng. The proposed method showed potential in providing comprehensive information on the nutrient content of foods.