Change of oil-degrading activity in microorganisms stored under laboratory conditions

Change of the oil-degrading activity was studied in psychrophilic microbial strains Rhodococcus spp. DS-07, DS-21 and Pseudomonas spp. DS-09, DS-22 maintained on various media: rich and synthetic with a selective agent. After 2.5 years of storage on rich medium, the oil-degrading activity decreased...

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Published inApplied biochemistry and microbiology Vol. 44; no. 3; pp. 282 - 286
Main Authors Belousova, N. I., Baryshnikova, L. M., Shkidchenko, A. N.
Format Journal Article
LanguageEnglish
Published Dordrecht SP MAIK Nauka/Interperiodica 01.05.2008
Springer Nature B.V
Subjects
Biochemistry
Biodegradation
Biomedical and Life Sciences
Bioremediation
Life Sciences
Medical Microbiology
Microbiology
Microorganisms
Oil
Pseudomonas
Rhodococcus
Studies
Rhodococcus
Synthetic Medium
Rich Medium
Apply Biochemistry
Hexadecane
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Summary:Change of the oil-degrading activity was studied in psychrophilic microbial strains Rhodococcus spp. DS-07, DS-21 and Pseudomonas spp. DS-09, DS-22 maintained on various media: rich and synthetic with a selective agent. After 2.5 years of storage on rich medium, the oil-degrading activity decreased by 50–60%, whereas this decrease was insignificant in the medium with oil. Passages to selective medium with oil after the storage partly restored the activity. It was found that storage of oil-degrading microorganisms caused loss of biodegradation plasmids. Their recovery and long-term preservation demand the presence of the selective agent in the medium.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683808030095