Bacteriocins as natural weapon against cancer: in vitro, in vivo, and in silico perspectives

• Published in: Discover applied sciences Vol. 7; no. 9; pp. 940 - 15
• Main Authors: Assega, Birhan Getie, Tsegaye, Kindu Nibret, Mitiku, Temesgen, Tsehai, Berhanu Andualem
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.09.2025; Springer Nature B.V; Springer
• Subjects: Angiogenesis; Animal models; Anticancer; Anticancer properties; Antimicrobial peptides; Antineoplastic drugs; Apoptosis; Applied and Technical Physics; Bacteria; Bacteriocin; Bacteriocins; Biocompatibility; Biosynthesis; Cancer cells; Cancer therapies; Cell cycle; Cell death; Cell differentiation; Cell proliferation; Chemistry/Food Science; Chemotherapy; Cytotoxicity; Earth Sciences; Engineering; Environment; Enzymes; Gram-negative bacteria; Gram-positive bacteria; Immune response; In vitro; In vitro methods and tests; In vivo; In vivo methods and tests; Lipid composition; Lipids; Materials Science; Membranes; Metastases; Metastasis; Molecular weight; Peptides; Proteins; Review; Side effects; Therapeutic applications; Toxicity; In vivo; Bacteriocin; Cancer cells; Cytotoxicity; In silico; Anticancer; In vitro
• ISSN: 3004-9261; 2523-3963; 3004-9261; 2523-3971
• DOI: 10.1007/s42452-025-06929-y

Background Cancer, characterized by uncontrolled cell proliferation, metastasis, and angiogenesis, remains a leading cause of death worldwide. Conventional cancer therapies leading to significant side effects. Consequently, there is an urgent need for alternative, targeted treatments. Bacteriocins, ribosomally synthesized antimicrobial peptides produced by bacteria, have emerged as promising anticancer agents. They exhibit selective toxicity towards cancer cells, owing to their cationic, amphiphilic, and membrane-active properties, which target the negatively charged membranes of cancer cells. Objective This review aims to explore bacteriocins as alternative anticancer agents, focusing on their mechanisms of action, discuss the challenges associated with their use, and explore future perspectives for their application in cancer therapy. Methods Recent in vitro, in vivo, and in silico studies on bacteriocins' anticancer effects are reviewed. Bacteriocins' ability to differentiate cancer cells from normal cells, their mechanisms of action, and their potential for clinical application were assessed. Results Bacteriocins demonstrate anticancer properties by exploiting differences between cancer and normal cells, including membrane charge, lipid composition, and metabolic conditions. They induce apoptosis, disrupt mitochondrial integrity, affect angiogenesis, and modulate immune responses. In vitro studies show promising results with bacteriocins like nisin, inhibiting cancer cell growth and inducing apoptosis. In vivo studies, such as those with microcin E492, demonstrate significant tumor reduction in animal models. Docking analyses suggest strong binding affinities between bacteriocins and cancer-related targets. Conclusion Bacteriocins are promising tools for targeted cancer therapy with minimal side effects on healthy cells. They may enhance existing cancer therapies by inducing apoptosis and improving immune responses. Overcoming challenges to use bacteriocins as anticancer agents and exploring their potential in clinical setting is crucial. Article Highlights Bacteriocins exhibit selective toxicity towards cancer cells by exploiting their unique membrane properties. Bacteriocins enhance existing cancer treatments, showing promise in combination therapies. Stability and large-scale production barriers must be addressed for clinical application.