Gramicidin A interaction at a dioleoyl phosphatidylcholine monolayer on a mercury drop electrode

A biosensor where the sensing surface is a fluid dioleyl phosphatidylcholine monolayer (DOPC) deposited on a mercury drop was used. The lipid monolayer was held in 0.1 M NaCl and a concentration of gramicidin A in the range 0-12 nM was used. Electrochemical impedance spectroscopy in the frequency ra...

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Bibliographic Details
Published inAnalytical and bioanalytical chemistry Vol. 375; no. 3; pp. 350 - 355
Main Authors Lindholm-Sethson, Britta, Nyström, Josefina, Geladi, Paul, Nelson, Andrew
Format Journal Article
LanguageEnglish
Published Germany 01.02.2003
Subjects
Biosensing Techniques - methods
Electric Capacitance
Electric Impedance
Electrochemistry - methods
Electrodes
Gramicidin - analysis
Gramicidin - chemistry
Lipid Bilayers - chemistry
Mercury
Multivariate Analysis
Phosphatidylcholines - chemistry
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Summary:A biosensor where the sensing surface is a fluid dioleyl phosphatidylcholine monolayer (DOPC) deposited on a mercury drop was used. The lipid monolayer was held in 0.1 M NaCl and a concentration of gramicidin A in the range 0-12 nM was used. Electrochemical impedance spectroscopy in the frequency range 0.1-65 kHz was employed to investigate how the defect-free monolayer responds to interactions of gramicidin A in solution. The data was analyzed both with multivariate data analysis and classical electrochemical methods. The principal component analysis of the resulting impedance spectra gave a linear dependence on the concentration of gramicidin A. An increasing permittivity was observed in the low-frequency regime with increasing concentration of gramicidin A in solution.
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ISSN:1618-2642
DOI:10.1007/s00216-002-1697-9