The effect of choline chloride-butanediol based deep eutectic solvents on ultrasound-assisted extraction, antioxidant activity and stability of anthocyanins extracted from Perilla frutescens (L.) Britt

• Published in: Sustainable chemistry and pharmacy Vol. 32; p. 101000
• Main Authors: Bu, Fanzhen, Zhao, Yanqiao, Li, Bin, Zhang, Xin, Li, Jianying
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2023
• Subjects: Anthocyanins; Deep eutectic solvents; Perilla leaves; Ultrasonic assisted extraction; Perilla leaves; Anthocyanins; Deep eutectic solvents; Ultrasonic assisted extraction
• ISSN: 2352-5541; 2352-5541
• DOI: 10.1016/j.scp.2023.101000

To explore the relation between anthocyanins extraction performance and physicochemical properties of choline chloride (ChCl)-butanediol deep eutectic solvents (DESs), sixty formulas consisting of ChCl with four butanediol isomers (1,3-butanediol, 1,4-butanediol, 2,3-butanediol, or 1,2-butanediol) at different molar ratios (ChCl/butanediol) and different amounts of water were examined. In this study, 1,3-butanediol was selected as the most promising hydrogen bond donor for the preparation of DESs. The highest anthocyanins content (9.97 ± 0.04 mg/g) was obtained using the DES composed of ChCl and 1,3-butanediol with 1:2 M ratio containing 50% water (DES-2 (50%)) coupled with ultrasound assisted extraction (UAE). The anthocyanins extracted by DES-2 (50%) presented highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) antioxidation activity (1.88 ± 0.01 μM TE/g DW), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) antioxidation activity (5.10 ± 0.09 μM TE/g DW), ferric reducing/antioxidant power (FRAP) antioxidation activity (28.39 ± 0.21 μM TE/g DW). Comparative investigations on stability of anthocyanins proved that DES-2 (50%) offers outstanding advantages over acidified ethanol. After storage at 4°C for 6 days, the remaining fraction of the anthocyanins extracted by DES-2 (50%) (92.01 ± 0.52%) was higher than anthocyanins extracted by ethanol (84.25 ± 0.57%). [Display omitted] •Water plays an important role in extraction by deep eutectic solvents (DESs).•Sixty systems were studied with choline chloride and four butanediol isomers.•The highest extraction amount of anthocyanins by DESs was 9.97 ± 0.04 mg/g.•Anthocyanins extracted by DESs showed superior antioxidant and stability activities.