What about computational super-resolution in fluorescence Fourier light field microscopy?

Recently, Fourier light field microscopy was proposed to overcome the limitations in conventional light field microscopy by placing a micro-lens array at the aperture stop of the microscope objective instead of the image plane. In this way, a collection of orthographic views from different perspecti...

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Bibliographic Details
Published inOptics express Vol. 28; no. 11; pp. 16554 - 16568
Main Authors Stefanoiu, Anca, Scrofani, Gabriele, Saavedra, Genaro, Martínez-Corral, Manuel, Lasser, Tobias
Format Journal Article
LanguageEnglish
Published 25.05.2020
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Summary:Recently, Fourier light field microscopy was proposed to overcome the limitations in conventional light field microscopy by placing a micro-lens array at the aperture stop of the microscope objective instead of the image plane. In this way, a collection of orthographic views from different perspectives are directly captured. When inspecting fluorescent samples, the sensitivity and noise of the sensors are a major concern and large sensor pixels are required to cope with low-light conditions, which implies under-sampling issues. In this context, we analyze the sampling patterns in Fourier light field microscopy to understand to what extent computational super-resolution can be triggered during deconvolution in order to improve the resolution of the 3D reconstruction of the imaged data.
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ISSN:1094-4087
1094-4087
DOI:10.1364/OE.391189