rapid DNA minipreparation method suitable for AFLP and other PCR applications

A rapid DNA minipreparation method was developed for rice and other plant species. This method uses an Eppendorf tube and 1-ml pipette tip to grind plant tissues, and requires only one transfer for DNA isolation. In a single day, one person can complete DNA isolation from more than 120 leaf samples....

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Bibliographic Details
Published inPlant molecular biology reporter Vol. 17; no. 1; pp. 53 - 57
Main Authors Chen, D.H, Ronald, P.C
Format Journal Article
LanguageEnglish
Published New York Springer Nature B.V 01.01.1999
Subjects
Amplified fragment length polymorphism
Deoxyribonucleic acid
DNA
genetic markers
genetic techniques and protocols
Genetic testing
isolation
Leaves
methodology
Oryza sativa
Plant species
Plant tissues
Polymerase chain reaction
rapid methods
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Summary:A rapid DNA minipreparation method was developed for rice and other plant species. This method uses an Eppendorf tube and 1-ml pipette tip to grind plant tissues, and requires only one transfer for DNA isolation. In a single day, one person can complete DNA isolation from more than 120 leaf samples. The yields of the DNA samples ranged from 2.3 to 5.2 μg from 25-50 mg fresh leaf tissue. DNA samples extracted using this method from rice were completely digested with five restriction enzymes (EcoR I, EcoR V, Hind III, Mse I and Pst I) and were successfully used for AFLP and other PCR applications.
ISSN:0735-9640
1572-9818
DOI:10.1023/A:1007585532036