Identification of suitable reference gene for quantitative transcription analysis (RT-qPCR) of Fusarium culmorum genes in infected barley plants

•Correct determination of Fusarium culmorum gene transcription in infected barley.•Determination of suitable reference gene for qRT-PCR.•Fusarium UBC is identified as suitable reference gene for infected plant material.

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Bibliographic Details
Published inJournal of cereal science Vol. 79; pp. 418 - 423
Main Authors Faltusová, Zuzana, Pavel, Jozef, Vaculová, Kateřina, Chrpová, Jana, Ovesná, Jaroslava
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.01.2018
Subjects
Fusarium
Reference gene
RT -qPCR
UBC
Fusarium
RT -qPCR
Reference gene
UBC
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Summary:•Correct determination of Fusarium culmorum gene transcription in infected barley.•Determination of suitable reference gene for qRT-PCR.•Fusarium UBC is identified as suitable reference gene for infected plant material.
ISSN:0733-5210
1095-9963
DOI:10.1016/j.jcs.2017.12.004