Helix pomatia A hemagglutinin, a surface marker for bovine T-lymphocytes

Bovine peripheral blood lymphocytes (PBL) were isolated from blood collected from 6 cattle. After treatment with neuraminidase, 40 or 60% of the cells were shown to combine with Helix Pomatia A hemagglutinin (HP) depending whether a direct or indirect fluorescence technique was used. About 20% of th...

Full description

Saved in:
Bibliographic Details
Published inVeterinary immunology and immunopathology Vol. 1; no. 1; pp. 27 - 36
Main Authors Morein, B., Hellström, U., Axelsson, L-G., Johansson, C., Hammarström, S.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.11.1979
Subjects
animal health
Animals
Biomarkers - blood
Cattle - immunology
Cell Separation - methods
Helix (Snails)
Hemagglutinins - immunology
Lectins - immunology
Rosette Formation
T-Lymphocytes - immunology
veterinary medicine
Online AccessGet full text

Cover

More Information
Summary:Bovine peripheral blood lymphocytes (PBL) were isolated from blood collected from 6 cattle. After treatment with neuraminidase, 40 or 60% of the cells were shown to combine with Helix Pomatia A hemagglutinin (HP) depending whether a direct or indirect fluorescence technique was used. About 20% of the cells were Ig-bearing. With double staining fluorescence technique, it was shown that cells attaching to HP were not Ig-bearing and the reverse. With the aid of HP, covalently bound to Sepharose, Ig-bearing cells could be separated from cell populations attaching to HP. The fraction of cells forming rosettes with sheep erythrocytes was proportional to that of HP attaching cells both before and after fractionation on the HP column. It is therefore concluded that HP is a marker for bovine T-cells, and that this lectin may be used to separate B-cells from T-cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0165-2427
1873-2534
DOI:10.1016/0165-2427(79)90005-9