Simple fabrication of ZnO/Pt/chitosan electrode for enzymatic glucose biosensor

• Published in: Sensors and actuators. B, Chemical Vol. 202; pp. 827 - 833
• Main Authors: Anusha, J.R., Kim, Hee-Je, Fleming, Albin T., Das, S. Jerome, Yu, Kook-Hyun, Kim, Byung Chul, Raj, C. Justin
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 31.10.2014
• Subjects: Cyclic voltammetry; Glucose sensor; Impedance spectroscopy; Nanoparticle; Zinc oxide; Cyclic voltammetry; Zinc oxide; Glucose sensor; Impedance spectroscopy; Nanoparticle
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2014.06.024

•Zinc oxide/platinum/chitosan heterostructure was fabricated for glucose biosensor.•Pt nanoparticle layer over ZnO enhances the sensitivity and performance of the biosensor.•The electrode shows better catalytic activity and electron transfer properties. Enzymatic glucose biosensor was fabricated with the aid of zinc oxide (ZnO) porous nanostructure on FTO glass substrate by simple drop casting technique. By introducing a small layer of platinum (Pt) nanoparticles and chitosan (CS) over the ZnO porous nanostructure have enhanced the glucose sensing property of the fabricated biosensor to a higher order. The morphological and structural properties of the electrodes were analyzed using scanning electron microscopy and X-ray diffraction analysis. The glucose sensing properties of glucose oxidase (GOx) immobilized electrodes were estimated using electrochemical analysis. The ZnO/Pt/CS heterostructure conjugated with GOx shows good analytical performances with high sensitivity (62.14μAmM−1cm−2) in a wide linear range and low detection compared with the bare ZnO/CS/GOx biosensor. In addition, the catalytic properties of the electrodes in H2O2 solution was studied and discussed in detail using cyclic voltammetry and impedance spectroscopic techniques.