A system for ω-transaminase mediated (R)-amination using l -alanine as an amine donor

Chiral amines are important building blocks for fine chemicals and pharmaceuticals. Consequently, various biocatalytic routes in particular using omega -transaminases ( omega -TAs) have been developed recently. Although catalysts for the synthesis of both enantiomers are available, the application o...

Full description

Saved in:
Bibliographic Details
Published inGreen chemistry : an international journal and green chemistry resource : GC Vol. 17; no. 5; pp. 2952 - 2958
Main Authors Richter, N, Farnberger, JE, Pressnitz, D, Lechner, H, Zepeck, F, Kroutil, W
Format Journal Article
LanguageEnglish
Published 01.05.2015
Subjects
Alanine
Amines
Catalysis
Catalysts
Costs
Enantiomers
Fine chemicals
Synthesis
Online AccessGet full text

Cover

More Information
Summary:Chiral amines are important building blocks for fine chemicals and pharmaceuticals. Consequently, various biocatalytic routes in particular using omega -transaminases ( omega -TAs) have been developed recently. Although catalysts for the synthesis of both enantiomers are available, the application of alanine dependent (R)-selective omega -TAs is less favourable due to the requirement of the more expensive d-alanine as an amine donor. Here we describe an efficient method for (R)-amination using omega -TAs in combination with an alanine racemase (AlaR). In this case, the readily available l-alanine can be used as an amine donor leading to improved atom efficiency and significantly reduced costs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1463-9262
1463-9270
DOI:10.1039/c4gc02363c