Fed-batch culture of Oplopanax elatus adventitious roots: Establishment of a complete culture system

• Published in: Biochemical engineering journal Vol. 194; p. 108898
• Main Authors: Jin, Mei-Yu, Hao, Yue-Jun, Zhang, Ke-Xin, Yin, Cheng-Ri, Jiang, Jun, Piao, Xuan-Chun, Lian, Mei-Lan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2023
• Subjects: Adventitious roots; Fed-batch culture; Feeding strategy; Harvest time; Initial culture medium; Initial culture medium; Harvest time; Feeding strategy; Adventitious roots; Fed-batch culture
• ISSN: 1369-703X; 1873-295X
• DOI: 10.1016/j.bej.2023.108898

Fed-batch culture is rarely applied in plant organ culture, but it has been attempted to be used in Oplopanax elatus adventitious root (AR) culture. To establish a complete fed-batch O. elatus AR culture system, this study investigated the effects of sucrose, phosphorus, and nitrogen concentrations in the initial medium, and feeding time, feeding volume, and harvest time on AR biomass and flavonoid or polysaccharide accumulation. The results indicated the initial medium (3 L) containing 0.938 mM phosphorus and 40 mM nitrogen supplemented with 40 g/L sucrose, feeding with 2 L feeding medium on the 15th day, and harvesting on the 45th day were the most suitable conditions for the production of flavonoid- and polysaccharide-enriched ARs. In the established culture system, 76.7 mg/L/day of flavonoids and 200.9 mg/L/day of polysaccharides were obtained. This study suggested that fed-batch culture is a promising approach for mass production of O. elatus ARs. [Display omitted] •Fed-batch culture system of Oplopanax elatus adventitious roots is established.•Sucrose, phosphorus, and nitrogen concentrations in initial medium are optimized.•Suitable feeding time and feeding volume are selected.•Harvesting adventitious roots after 40 days of culture is the most optimal.•Fed-batch cultured adventitious roots contain rich flavonoids and polysaccharides.