Ru nanosheets with specific peroxidase activity for biosensor applications

• Published in: Sensors and actuators. B, Chemical Vol. 394; p. 134478
• Main Authors: Hu, Xinyan, Zhu, Tianbao, Zhang, Gongxin, Ye, Zichen, Chen, Xiaolan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2023
• Subjects: Colorimetric detection; Ethanol; Nanozyme; Peroxidase-like activity; Ru nanosheets; Uric acid; Nanozyme; Peroxidase-like activity; Ru nanosheets; Uric acid; Ethanol; Colorimetric detection
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2023.134478

Nanozymes displaying peroxidase-like activity are helpful for various biomedical applications. In this work, uniform triangular Ru nanosheets (NSs) prepared by a facile hydrothermal approach are first found to possess high performance and specific peroxidase (POD)-like activity which catalyzes the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) with H2O2 to blue oxidized TMB (oxTMB). On basis of their outstanding POD activity, we successfully developed two highly sensitive colorimetric methods for detections of ethanol (0.02–0.88 mM, R2 = 0.9909) and uric acid (UA) (3.91–500 μM, R2 = 0.9938) in serum under the optimal conditions. The detection limits were 0.011 mM and 3.05 μM for ethanol and UA, respectively. These Ru NSs-based biosensors possess prominent characteristics of simple operation, sensitivity, reliability and high selectivity, enabling them suitable for on-demand analysis. [Display omitted] •Uniform Ru NSs were prepared and their physicochemical properties were explored.•Ru NSs possessed a high-performance and specific POD activity.•Ru NSs-based biosensors for colorimetric assays of ethanol and uric acid were built.•The biosensors demonstrated broad linearity and high sensitivity.