Glucose Challenge Stimulates Reactive Oxygen Species (ROS) Generation by Leucocytes

• Published in: The journal of clinical endocrinology and metabolism Vol. 85; no. 8; pp. 2970 - 2973
• Main Authors: Mohanty, Priya, Hamouda, Wael, Garg, Rajesh, Aljada, Ahmad, Ghanim, Husam, Dandona, Paresh
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Oxford University Press 01.08.2000; Endocrine Society
• Subjects: Adult; Biological and medical sciences; Blood Glucose - physiology; Blood levels; Diabetes mellitus; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; Glucose; Glucose - pharmacology; Humans; In Vitro Techniques; Kinetics; Leukocytes; Leukocytes (mononuclear); Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - physiology; Male; Medical sciences; NAD(P)H oxidase; NADH Dehydrogenase - blood; NADPH Oxidases; Neutrophils - drug effects; Neutrophils - physiology; p47phox Protein; Phosphoproteins - blood; Reactive oxygen species; Reactive Oxygen Species - metabolism; Thiobarbituric Acid Reactive Substances - analysis; Vitamin E - blood; Endocrinopathy; Human; NADPH; Pathophysiology; Enzyme; Leukocyte; Diabetes mellitus; Oxidase; α-Tocopherol; Biosynthesis; Glucose; Protein; Food intake; Adult; Oxidoreductases
• ISSN: 0021-972X; 1945-7197
• DOI: 10.1210/jcem.85.8.6854

Diabetes mellitus is associated with increased ROS generation, oxidative injury and obesity. To elucidate the relationship between nutrition and ROS generation, we have investigated the effect of glucose challenge on ROS generation by leucocytes, p47phox protein, a key protein in the enzyme NADPH oxidase and α-tocopherol levels. Blood samples were drawn from 14 normal subjects prior to, at 1, 2 and 3 h following ingestion of 75 g glucose. ROS generation by polymorphonuclear leucocytes (PMNL) and mononuclear cells (MNC) increased to a peak of 244 ± 42% and 233 ± 34% of the basal respectively at 2 h. The levels of p47phox in MNC homogenates increased significantly at 2 h and 3 h after glucose intake. α-Tocopherol levels decreased significantly at 1 h, 2 h, and 3 h. We conclude that glucose intake stimulates ROS generation and p47phox of NADPH oxidase; increases oxidative load and causes a fall in α-tocopherol concentration.