Alteration of free amino acid concentrations in insect galls induced by Andricus mukaigawae (Hymenoptera; Cynipidae)

• Published in: Ecological entomology Vol. 45; no. 5; pp. 945 - 954
• Main Authors: Yang, Xiao‐Hui, Li, Xiang‐Mei, Zhu, Dao‐Hong
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.10.2020; Wiley Subscription Services, Inc
• Subjects: Adults; amino acid; Amino acids; Andricus; Andricus mukaigawae; Asparagine; Composition; Depletion; Galling; Galls; Hypotheses; insect gall; Insects; Larvae; Liquid chromatography; Nutrition; nutrition hypothesis; Proline; Tryptophan
• ISSN: 0307-6946; 1365-2311
• DOI: 10.1111/een.12867

1. Insect galls are abnormal plant growths that develop in response to a stimulus provided by a galling insect. The nutrition hypothesis suggests that the concentrations of nutritive compounds in galls are changed to provide optimum nutrition for the larvae and adults of galling insects. 2. To test the nutrition hypothesis, we determined the concentrations of 20 free amino acids in galls and in galled and ungalled twigs of Quercus fabri during the larval and adult stages of Andricus mukaigawae using high‐performance liquid chromatography with ultraviolet–visible detection. 3. At the larval stage, the concentrations of 12 out of 20 amino acids in A. mukaigawae galls were significantly higher than those in galled and ungalled twigs. Asparagine and tryptophan were the most abundant nonessential and essential amino acids, respectively, in A. mukaigawae galls. 4. At the adult stage, the concentrations of most amino acids, except proline, were significantly lower in A. mukaigawae galls than in galled and ungalled twigs. The A. mukaigawae adults may not manipulate amino acid levels because the adults do not feed on galls. The decrease of amino acid levels in adult galls may be viewed as a depletion. 5. The composition of free amino acids in A. mukaigawae galls was significantly different from the composition in galled and ungalled twigs in both the larval and adult stages. 6. Our results may support the nutrition hypothesis. We suggest that a high concentration of proline in A. mukaigawae galls may protect larvae and adults from plant defense responses. A. mukaigawa galls had higher concentration of total and most free amino acids than galled and ungalled twigs in the larva stage, and results were opposite in the adult stage. The composition of free amino acids in A. mukaigawae galls was obviously different from those in galled and ungalled twigs in both the larval and adult stages. Our results supported the nutrition hypothesis. Furthermore, we suggest that a high concentration of proline in A. mukaigawae galls could protect larvae and adults from plant defense responses.