Study of the efficacy of Aureobasidium strains belonging to three different species: A. pullulans, A. subglaciale and A. melanogenum against Botrytis cinerea of tomato

The difference in antagonistic activity against the causal agent of grey mould (Botrytis cinerea) of tomato between Aureobasidium strains belonging to three different species, namely A. pullulans, A. melanogenum and A. subglaciale, was evaluated by in vitro and in vivo assays. In the yeast–pathogen...

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Bibliographic Details
Published inAnnals of applied biology Vol. 177; no. 2; pp. 266 - 275
Main Authors Di Francesco, Alessandra, Di Foggia, Michele, Zajc, Janja, Gunde‐Cimerman, Nina, Baraldi, Elena
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.09.2020
Wiley Subscription Services, Inc
Subjects
Antibiotics
Aureobasidium
bio control
Biological control
Botrytis cinerea
Enzymes
Grey mold
Infrared spectroscopy
Lytic enzymes
Metabolites
Mycelia
non‐volatile metabolites
Pathogens
Pectinase
Polygalacturonase
Polysaccharides
Saccharides
Siderophores
Species
Tomatoes
Virulence
Xylanase
Yeasts
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Summary:The difference in antagonistic activity against the causal agent of grey mould (Botrytis cinerea) of tomato between Aureobasidium strains belonging to three different species, namely A. pullulans, A. melanogenum and A. subglaciale, was evaluated by in vitro and in vivo assays. In the yeast–pathogen direct interaction experiment, all the strains significantly reduced B. cinerea growth, with A. melanogenum the least efficient species (17.8% of reduction) compared to A. pullulans and subglaciale (22 and 27.8%). The non‐volatile metabolites produced by all three species reduced mycelial growth between 95 and 100%. These metabolites were characterised by FT‐IR spectroscopy as polysaccharides, lytic enzymes, siderophores and antibiotics. The inhibitory effect of Aureobasidium strains on pathogenic enzymes such as xylanase, polygalacturonase and pectinase was measured showing A. pullulans strains as capable of strong inhibition of xylanase, an enzyme directly related to the virulence of necrotrophic pathogens such as B. cinerea. Our data demonstrate that the different species of Aureobasidium isolated from a range of non‐conventional environments exerted variable efficacy against B. cinerea, with A. pullulans as the most active species followed by A. subglaciale and A. melanogenum as ineffective and not suitable for biocontrol applications.
ISSN:0003-4746
1744-7348
DOI:10.1111/aab.12627