Correction: Fast, quantitative, murine cardiac 19F MRI/MRS of PFCE-labeled progenitor stem cells and macrophages at 9.4T

• Published in: PloS one Vol. 14; no. 11; p. e0225786
• Main Authors: Constantinides, Christakis, Maguire, Mahon, McNeill, Eileen, Carnicer, Ricardo, Swider, Edyta, Srinivas, Mangala, Carr, Carolyn A., Schneider, Jurgen E.
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 21.11.2019; Public Library of Science (PLoS)
• Subjects: Analytical methods; Appendix; Butterflies; Cell number; Coils; Composite functions; Data acquisition; Dependence; Detection limits; Flow cytometry; Image detection; Linearity; Macrophages; Magnetic resonance imaging; Magnetic resonance spectroscopy; Medical imaging; Sensitivity; Stem cell transplantation; Stem cells; Tubes
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0225786

19F MRI-based quantification in solutions and CPCs and determination of cellular detectability limit. 19F MR spectroscopy, image-based quantification, and sensitivity detection limits: (A, B) Axial 1H and 19F images from TFA phantoms of different concentrations (25–100 mM), and images of a multivial sensitivity phantom containing 0.25, 0.5, 0.75, and 1 million labeled/transfected CT cells suspended in media for sensitivity limit detection (cell pellets resided at the bottom of the Eppendorf tubes) using the butterfly coil. The linearity of the evoked fully relaxed spectral area versus cell number was independently confirmed using fast, direct, image-based SPGR using CPCs (butterfly coil) (results not shown). https://doi.org/10.1371/journal.pone.0225786.g002 S1 Appendix erroneously contains annotated comments. In accordance to basic MRI principles, SNR can be formulated as ) , , , , ( 2 1 ,  TE TR T T S T V SNT total acq   where Δ V is the sample volume, and T acq,total is the total data acquisition time considered as the time during which the data acquisition window is open during each readout (equal to the number of frequency encoding steps, N freq , divided by the receiver bandwidth (BW)) [1], BW N T N T freq sampling freq total acq   . , T 1 and T 2 the respective relaxation values, and θ is the flip angle. ) , , , , ( 2 1  TE TR T T S is a composite function that determines the dependence of the evoked signal on relaxation times and pulse sequence acquisition parameters.