Occurrence of mastitis pathogens in relation to somatic cells

• Published in: Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis Vol. 61; no. 5; pp. 1505 - 1511
• Main Authors: Vyletelova Klimesova, M., Dairy Research Institute, Prague (Czech Republic), Hanus, O., Dairy Research Institute, Prague (Czech Republic), Hasonova, L., University of South Bohemia in Ceske Budejovice (Czech Republic). Agricultural Faculty, Roubal, P., Dairy Research Institute, Prague (Czech Republic), Manga, I., Agritec Plant Research, Ltd., Sumperk (Czech Republic), Nejeschlebova, L., Dairy Research Institute, Prague (Czech Republic)
• Format: Journal Article
• Language: English
• Published: Mendel University Press 2013
• Subjects: AGENT PATHOGENE; ANIMAL NUTRITION; ANTIBIOTICOS; ANTIBIOTICS; ANTIBIOTIQUE; BOVINE MASTITIS; CONTEO DE CELULAS SOMATICAS; COWS; cow’s milk; discrimination limit; ENFERMEDADES GLANDULAS MAMARIAS; ENSAYO; ENTEROBACTER; ENTEROCOCCUS; EQUIPO DE ORDENO; ESCHERICHIA COLI; ESTRES; EVALUACION; EVALUATION; GENETICA MOLECULAR; GENETIQUE MOLECULAIRE; GLANDE MAMMAIRE; GLANDULAS MAMARIAS; http://www.fao.org/aos/agrovoc#c_15967; http://www.fao.org/aos/agrovoc#c_16429; http://www.fao.org/aos/agrovoc#c_16605; http://www.fao.org/aos/agrovoc#c_1939; http://www.fao.org/aos/agrovoc#c_23918; http://www.fao.org/aos/agrovoc#c_25767; http://www.fao.org/aos/agrovoc#c_26709; http://www.fao.org/aos/agrovoc#c_2736; http://www.fao.org/aos/agrovoc#c_27577; http://www.fao.org/aos/agrovoc#c_27925; http://www.fao.org/aos/agrovoc#c_33554; http://www.fao.org/aos/agrovoc#c_33700; http://www.fao.org/aos/agrovoc#c_4140; http://www.fao.org/aos/agrovoc#c_4561; http://www.fao.org/aos/agrovoc#c_4562; http://www.fao.org/aos/agrovoc#c_492; http://www.fao.org/aos/agrovoc#c_5630; http://www.fao.org/aos/agrovoc#c_7377; http://www.fao.org/aos/agrovoc#c_7452; http://www.fao.org/aos/agrovoc#c_7683; http://www.fao.org/aos/agrovoc#c_7715; http://www.fao.org/aos/agrovoc#c_9378; LACTACION; LACTATION; MALADIE DES GLANDES MAMMAIRES; MAMMARY GLAND DISEASES; MAMMARY GLANDS; MAMMITE BOVINE; MASTITIS BOVINA; MATERIEL DE TRAITE; METHODE STATISTIQUE; METODOS ESTADISTICOS; MILKING EQUIPMENT; MOLECULAR GENETICS; NUMERATION CELLULAIRE SOMATIQUE; NUTRICION ANIMAL; NUTRITION ANIMALE; ORGANISMOS PATOGENOS; PATHOGENS; SOMATIC CELL COUNT; STAPHYLOCOCCUS AUREAS; STAPHYLOCOCCUS AUREUS; Staphylococcus haemolyticus; STATISTICAL METHODS; STREPTOCOCCUS FAECALIS; STREPTOCOCCUS UBERIS; STRESS; TERAPIA; TESTAGE; TESTING; THERAPEUTIQUE; THERAPY; VACA; VACHE
• ISSN: 1211-8516; 2464-8310
• DOI: 10.11118/actaun201361051505

There were examined 161 cows from 4 farms in total. The suspect animals were selected according to viscosity test results, clinical symptoms and somatic cell count (SCC). Milk samples were examined for the presence of pathogens and for SCC. 55 mastitis pathogens were identified. The most frequently isolated species was Enterococcus faecalis (n = 20), followed by Staphylococcus aureus (n = 6) and Streptococcus uberis (n = 5). The SCC ranged from 9 to 24 204 ths/ml. There was positive occurrence of bacteria genus Staphylococcus and Enterococcus at lower SCC (50 ths/ml) and at higher SCC numbers (more than 300 ths/ ml) bacteria genus Streptococcus, Enterobacter and Escherichia coli. Differences in SCC were significant in negative samples xg 131 SCC versus 491 for positive, 611 for staphylococci and 464 ths/ml for other positive. SCC discrimination limit for practical likelihood of pathogen occurrence estimation in infectious sample groups was calculated. This limit for suspicion of infection is 159 for positive group, 113 for staphylococci and 174 ths/ml for other positive. This could be possible to recommend the value 174 ths/ml for practical use with target to apply preventive or curative measures.