Isolation, characterization, and propagation in vitro of human glomerular endothelial cells

Human glomerular endothelial cells have been isolated, cloned, and characterized. They appeared as the first outgrowth from human glomeruli in the presence of platelet-derived growth factor, which was also a requirement for continuous growth. By phase microscopy they appeared as monolayers of polygo...

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Published inThe Journal of experimental medicine Vol. 160; no. 1; pp. 323 - 328
Main Authors Striker, G E, Soderland, C, Bowen-Pope, D F, Gown, A M, Schmer, G, Johnson, A, Luchtel, D, Ross, R, Striker, L J
Format Journal Article
LanguageEnglish
Published United States The Rockefeller University Press 01.07.1984
Subjects
Cell Division
Cell Separation - methods
Endothelium - cytology
Endothelium - enzymology
Endothelium - ultrastructure
Fluorescent Antibody Technique
Humans
Kidney Glomerulus - cytology
Kidney Glomerulus - enzymology
Kidney Glomerulus - ultrastructure
Peptidyl-Dipeptidase A - metabolism
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Summary:Human glomerular endothelial cells have been isolated, cloned, and characterized. They appeared as the first outgrowth from human glomeruli in the presence of platelet-derived growth factor, which was also a requirement for continuous growth. By phase microscopy they appeared as monolayers of polygonal cells. Von Willebrand's factor (VWF) was detected in the cytoplasm of all clones. Their intermediate filaments differed antigenically from that present in human umbilical vein endothelial cells. Like other endothelial cells, they demonstrated high levels of membrane-associated angiotensin-converting enzyme (ACE).
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-1007
1540-9538
DOI:10.1084/jem.160.1.323