Construction of a novel fluorescent probe for sensitive determination of glyphosate in food and imaging living cells

• Published in: Analytical methods Vol. 16; no. 21; pp. 3364 - 3371
• Main Authors: Lin, Xiaoping, Chen, Taiyi, Hu, Jiayun, Mao, Xiaoqiong, Liu, Mengqing, Zeng, Rongying, Zhong, Qingmei, Chen, Wen
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 30.05.2024
• Subjects: Agrochemicals; Cells (biology); Chelation; Copper; Copper - analysis; Copper - chemistry; Fluorescent Dyes - chemistry; Fluorescent indicators; Food Analysis - methods; Food Contamination - analysis; Glycine - analogs & derivatives; Glycine - chemistry; Glyphosate; Herbicides; Herbicides - analysis; Herbicides - chemistry; Horticulture; Humans; Imaging; Interference immunity; Limit of Detection; Optical Imaging - methods; Sensitivity; Smartphone; Spectrometry, Fluorescence - methods
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/d4ay00380b

Glyphosate is a widely used broad-spectrum herbicide in agriculture and horticulture to control a variety of weeds and undesirable plants. However, the excessive use of glyphosate has raised a number of environmental and human health concerns. It is urgent to develop tools to detect glyphosate. Herein, a novel dual-signal probe CCU-Cu 2+ was designed and synthesized on the basis of CCU. CCU exhibited excellent selectivity and great sensitivity for Cu 2+ which were based on both fluorescence "turn-off" reaction and comparative color visualisation methods. Due to the strong chelating ability of glyphosate on Cu 2+ , the CCU-Cu 2+ complex was applied to glyphosate detection in practical samples. The experimental results in vitro showed that the CCU-Cu 2+ complex was highly selective and rapid, with a low detection limit (1.6 μM), and could be recognised by the naked eye in the detection of glyphosate. Based on the excellent properties of the CCU-Cu 2+ complex, we also constructed a smartphone-assisted detection sensing system for glyphosate detection, which has the advantages of precision, sensitivity, and high interference immunity. Moreover, the CCU-Cu 2+ complex was also successfully employed for exogenous glyphosate imaging in living cells. These characteristics demonstrated that CCU-Cu 2+ holds significant potential for detection and imaging of glyphosate in bio-systems. A novel dual-signal fluorescent probe for sensitive determination of glyphosate in food and imaging living cells.