Quantitative Bacteriology of Amniotic Fluid from Women with Clinical Intraamniotic Infection at Term

• Published in: The Journal of infectious diseases Vol. 145; no. 1; pp. 1 - 8
• Main Authors: Gibbs, Ronald S., Blanco, James E., St. Clair, Patricia J., Castaneda, Yolanda S.
• Format: Journal Article
• Language: English
• Published: United States The University of Chicago Press 01.01.1982; University of Chicago Press
• Subjects: Adolescent; Adult; Amniotic fluid; Amniotic Fluid - microbiology; Anaerobic bacteria; Bacteria; Bacterial Infections - complications; Bacterial Infections - microbiology; Bacteroides - isolation & purification; Bacteroides - pathogenicity; Bacteroides Infections - complications; Bacteroides Infections - microbiology; Blood; Chorioamnionitis; Clinical Investigation; Escherichia coli - isolation & purification; Escherichia coli - pathogenicity; Escherichia coli Infections - complications; Escherichia coli Infections - microbiology; Female; Humans; Infections; Leukocytes; Leukocytosis - complications; Leukocytosis - microbiology; Obstetric Labor Complications - microbiology; Pregnancy; Specimens; Streptococcal Infections - complications; Streptococcal Infections - microbiology; Streptococcus agalactiae - isolation & purification; Streptococcus agalactiae - pathogenicity; Traumatic ruptures; Virulence; Women
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/145.1.1

Amniotic fluid was collected through an intrauterine catheter from 52 women with clinical intraamniotic infection and from 52 uninfected matched control women. The amniotic fluid was cultured quantitatively for anaerobes and aerobes. Patients with intraamniotic infection were matched with the control women on the basis of gestational age, interval from membrane rupture to specimen collection, and interval from membrane rupture to delivery. The patients with intraamniotic infection had a significantly higher mean temperature (38.4 vs. 37.1 C) and a higher mean leukocyte count (15,740 vs. 11,740 cells/mm3). In 80.6070 of specimens from the women with intraamniotic infection and 30.8% of those from the control subjects, ≥102 colony-forming units (cfu)/ ml were isolated from the amniotic fluid (P< 0.001). Also, in 69.2% of the former and 7.7% of the latter, there were ≥102 cfu of isolates considered to be “high-virulence” isolates/ml (P < 0.001).