Model phase separations of proteins using aqueous/ethanol components

Of several saturated or partially saturated inorganic salt solutions forming stable biphasic systems when mixed with ethanol, K sub(2)HPO sub(4)/ethanol was used to partition several model proteins, including lysozyme, BSA, chymotrypsinogen and the DNA polymerase from Thermus aquaticus. Partitioning...

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Bibliographic Details
Published inBiotechnology techniques Vol. 12; no. 5; pp. 363 - 365
Main Author LOUWRIER, A
Format Journal Article
LanguageEnglish
Published London Chapman and Hall 01.05.1998
Subjects
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Others
Various methods and equipments
Potassium phosphate
Biphasic system
Ethanol
Partition method
Partition coefficient
Aqueous solution
Protein
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Summary:Of several saturated or partially saturated inorganic salt solutions forming stable biphasic systems when mixed with ethanol, K sub(2)HPO sub(4)/ethanol was used to partition several model proteins, including lysozyme, BSA, chymotrypsinogen and the DNA polymerase from Thermus aquaticus. Partitioning was pH-dependent, and was successfully used for protein isolation from a mixture. Varying degrees of enzymatic activities were maintained.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1023/A:1008818229903