Imidazolium-based mass tags for protein biomarker detection using laser desorption ionization mass spectrometry

Novel imidazolium-based mass tags (IMTs) were designed, synthesized and applied to simultaneous in situ analysis of multiple biomarkers on less than 10 cells. The high sensitivity, flexible extensibility and excellent distinguishability of IMTs open new avenues for designing common mass tag template...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 59; no. 66; pp. 9996 - 9999
Main Authors Liu, Mingxia, Xue, Jinjuan, Liu, Huwei, Bai, Yu
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 15.08.2023
Subjects
Biomarkers
Immunoassay
Mass spectrometry
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Summary:Novel imidazolium-based mass tags (IMTs) were designed, synthesized and applied to simultaneous in situ analysis of multiple biomarkers on less than 10 cells. The high sensitivity, flexible extensibility and excellent distinguishability of IMTs open new avenues for designing common mass tag templates suitable for mass spectrometric immunoassay and provide an ideal option for multiplex-sensitive detection at the cellular scale. Novel imidazolium-based mass tags (IMTs) were designed, synthesized and applied to simultaneous in situ analysis of multiple biomarkers on less than 10 cells.
Bibliography:https://doi.org/10.1039/d3cc02907g
Electronic supplementary information (ESI) available: Synthetic procedures and characterisation of imidazolium-based mass tags, detection of cell surface proteins, LDI-MS spectra of Au-IMT1, fragment structures of IMT1 and CEA protein pre-incubation assay. See DOI
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/d3cc02907g