Imidazolium-based mass tags for protein biomarker detection using laser desorption ionization mass spectrometry
Novel imidazolium-based mass tags (IMTs) were designed, synthesized and applied to simultaneous in situ analysis of multiple biomarkers on less than 10 cells. The high sensitivity, flexible extensibility and excellent distinguishability of IMTs open new avenues for designing common mass tag template...
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Published in | Chemical communications (Cambridge, England) Vol. 59; no. 66; pp. 9996 - 9999 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Royal Society of Chemistry
15.08.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Novel imidazolium-based mass tags (IMTs) were designed, synthesized and applied to simultaneous
in situ
analysis of multiple biomarkers on less than 10 cells. The high sensitivity, flexible extensibility and excellent distinguishability of IMTs open new avenues for designing common mass tag templates suitable for mass spectrometric immunoassay and provide an ideal option for multiplex-sensitive detection at the cellular scale.
Novel imidazolium-based mass tags (IMTs) were designed, synthesized and applied to simultaneous
in situ
analysis of multiple biomarkers on less than 10 cells. |
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Bibliography: | https://doi.org/10.1039/d3cc02907g Electronic supplementary information (ESI) available: Synthetic procedures and characterisation of imidazolium-based mass tags, detection of cell surface proteins, LDI-MS spectra of Au-IMT1, fragment structures of IMT1 and CEA protein pre-incubation assay. See DOI ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1359-7345 1364-548X |
DOI: | 10.1039/d3cc02907g |