Single-atom nanozymes with intelligent response to pathological microenvironments for bacterially infected wound healing

• Published in: Biomaterials science Vol. 13; no. 4; pp. 133 - 144
• Main Authors: Wan, Zhen, Liu, Qingshan, Zhe, Yadong, Li, Jiarong, Ding, Danqi, Liu, Shuangjie, Wang, Hao, Qiao, Huanhuan, Yang, Jiang, Zhang, Shaofang, Mu, Xiaoyu
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 11.02.2025
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Bacteria; Biocompatibility; Humans; Mice; Nanostructures - chemistry; Reactive Oxygen Species - metabolism; Skin - drug effects; Thickness; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Wound healing; Wound Healing - drug effects; Wound Infection - drug therapy
• ISSN: 2047-4830; 2047-4849; 2047-4849
• DOI: 10.1039/d4bm01371a

Wound healing is a complex and dynamic process often accompanied by bacterial infection, inflammation, and excessive oxidative stress. Single-atom nanozymes with multi-enzymatic activities show significant potential for promoting the healing of infected wounds by modulating their antibacterial and anti-inflammatory properties in response to the wound's physiological environment. In this study, we synthesized MN 4 single-atom nanozymes with multi-enzymatic activities that intelligently respond to pH value changes in the wound healing process. In vitro experiments confirm their effectiveness against Gram-negative bacteria, attributed to elevated reactive oxygen species (ROS) accumulation within the bacterial cells. Moreover, a full-thickness skin wound-infected model demonstrates that MN 4 single-atom nanozymes accelerate wound repair and skin regeneration by suppressing the expression of tumor necrosis factor-alpha (TNF-α), promoting angiogenesis, and enhancing collagen deposition. In vivo biocompatibility experiments further demonstrate the favorable biocompatibility of these nanozymes, highlighting their potential for clinical applications in infected wound healing. These nanozymes respond intelligently to different microenvironments and may be suitable for addressing further complex and variable diseases. MN 4 single-atom nanozymes were synthesized and exhibited multi-enzymatic activities that promote infected wound healing by suppressing the expression of tumor necrosis factor-alpha, improving angiogenesis, and enhancing collagen deposition.