Identification of malaria-infected mosquitoes by a two-site enzyme-linked immunosorbent assay

A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two-site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and...

Full description

Saved in:
Bibliographic Details
Published inThe American journal of tropical medicine and hygiene Vol. 33; no. 2; pp. 227 - 231
Main Authors Burkot, T.R, Zavala, F, Gwadz, R.W, Collins, F.H, Nussenzweig, R.S
Format Journal Article
LanguageEnglish
Published United States 01.03.1984
Subjects
Animals
Anopheles - parasitology
Antibodies, Monoclonal
Antigens - analysis
disease diagnosis
Enzyme-Linked Immunosorbent Assay
enzymes
immunity
Immunoenzyme Techniques
labeling techniques
Plasmodium - immunology
Plasmodium - isolation & purification
Plasmodium berghei
Plasmodium berghei - immunology
Plasmodium berghei - isolation & purification
Plasmodium knowlesi
protozoal infections
serodiagnosis
Species Specificity
Vectors, Insecta
Online AccessGet more information

Cover

More Information
Summary:A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two-site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.1984.33.227