A novel EI-GC/MS method for the accurate quantification of anti-aging compound oleoylethanolamine in C. elegans

Endocannabinoids and related N -acyl ethanolamine-derived lipids affect a diverse array of physiological processes and pathological conditions. In the roundworm C. elegans , several endocannabinoid-like molecules have been implicated in regulating axon regeneration, energy balance and food intake as...

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Bibliographic Details
Published inAnalytical methods Vol. 10; no. 22; pp. 2551 - 2559
Main Authors Annibal, Andrea, Karalay, Özlem, Latza, Christian, Antebi, Adam
Format Journal Article
LanguageEnglish
Published Cambridge Royal Society of Chemistry 01.01.2018
Subjects
Aging
Cannabinoids
Collision dynamics
Energy balance
Ethanolamine
Food intake
Gas chromatography
Ionization
Life extension
Lipids
Molecular chains
Nematodes
Pharmacology
Quantitation
Regeneration
Signal transduction
Transduction
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Summary:Endocannabinoids and related N -acyl ethanolamine-derived lipids affect a diverse array of physiological processes and pathological conditions. In the roundworm C. elegans , several endocannabinoid-like molecules have been implicated in regulating axon regeneration, energy balance and food intake as well as aging. One such molecule oleoylethanolamine (OEA) has been shown to promote life extension through nuclear receptor signal transduction, and its accurate quantitation therefore is of high interest. Using a combination of electron impact ionization (EI) and collision induced dissociation coupled to gas chromatography (GC), we found unique fragmentation ions of OEA and designed a specific MRM method for its accurate quantification. Our method should provide a reproducible and robust way to measure OEA dynamics under different genetic, pharmacologic and environmental perturbations.
ISSN:1759-9660
1759-9679
DOI:10.1039/C8AY00396C