Some comparative properties of pyruvate kinase in haematopoietic cells and erythrocytes from rainbow trout ( Salmo gairdneri R.)

1. 1. Temperature acts as a pyruvate kinase regulator in haematopoietic cells and erythrocytes. 2. 2. Fructose-1,6-biphosphate and alanine act as allosteric modulators of pyruvate kinase in haematopoietic cells, while in erythrocytes although fructose-1,6-biphosphate exerts also allosteric effect, a...

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Published inComparative biochemistry and physiology. B, Comparative biochemistry Vol. 74; no. 4; pp. 801 - 805
Main Authors Gaitán, Soledad, Tejero, Concepción, Ruiz-Amil, Manuel
Format Journal Article
LanguageEnglish
Published England Elsevier Inc 1983
Subjects
Adenosine Triphosphate - pharmacology
Alanine - pharmacology
animal physiology
Animals
aquatic organisms
Erythrocytes - enzymology
Fructosediphosphates - pharmacology
Hematopoietic System - enzymology
Hydrogen-Ion Concentration
Kinetics
Pyruvate Kinase - analysis
Salmonidae - metabolism
Temperature
Trout - metabolism
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Summary:1. 1. Temperature acts as a pyruvate kinase regulator in haematopoietic cells and erythrocytes. 2. 2. Fructose-1,6-biphosphate and alanine act as allosteric modulators of pyruvate kinase in haematopoietic cells, while in erythrocytes although fructose-1,6-biphosphate exerts also allosteric effect, alanine appears to be a competitive inhibitor. ATP (1.0 mM) does not exert any clear effect on pyruvate kinase of both cellular populations. 3. 3. The level of specific activity of pyruvate kinase in haematopoietic cells is 40-fold that of PK from erythrocytes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0305-0491
DOI:10.1016/0305-0491(83)90149-9