Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches

Here we report two novel synthetic riboswitches that respond to ASP2905 and theophylline and function in reconstituted cell-free protein synthesis (CFPS) system. We encapsulated the CFPS system as well as DNA-templated encoding reporter genes regulated by these orthogonal riboswitches inside liposom...

Full description

Saved in:
Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 6; no. 46; pp. 5972 - 5975
Main Authors Ishii, Yuta, Fukunaga, Keisuke, Cooney, Aileen, Yokobayashi, Yohei, Matsuura, Tomoaki
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 04.06.2024
Subjects
Artificial Cells - chemistry
Artificial Cells - metabolism
Cell-Free System
Gene expression
Gene Expression Regulation
Genes, Reporter
Ligands
Liposomes - chemistry
Protein Biosynthesis
Protein synthesis
Riboswitch
Theophylline
Theophylline - chemistry
Online AccessGet full text

Cover

More Information
Summary:Here we report two novel synthetic riboswitches that respond to ASP2905 and theophylline and function in reconstituted cell-free protein synthesis (CFPS) system. We encapsulated the CFPS system as well as DNA-templated encoding reporter genes regulated by these orthogonal riboswitches inside liposomes, and achieved switchable and orthogonal control over gene expression by external stimulation with the cognate ligands. Here we report two novel synthetic riboswitches that respond to ASP2905 and theophylline and function in reconstituted cell-free protein synthesis (CFPS) systems.
Bibliography:https://doi.org/10.1039/d4cc00965g
Electronic supplementary information (ESI) available. See DOI
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/d4cc00965g