Anaerobiosis-induced virulence of Salmonella enterica subsp. enterica serovar Typhimurium: role of phospholipase Cγ signalling cascade

Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) can initiate entry into non-phagocytic epithelial cells by triggering certain signal transduction pathways, thereby allowing the pathogen to invade and establish a niche within host cells. Anaerobiosis has been shown to be an i...

Full description

Saved in:
Bibliographic Details
Published inJournal of medical microbiology Vol. 52; no. 9; pp. 741 - 745
Main Authors KHULLAR, Madhu, RAMAN DEEP SINGH, SMRITI, Manu, NIRMAL KUMAR GANGULY
Format Journal Article
LanguageEnglish
Published Reading Society for General Microbiology 01.09.2003
Subjects
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Salmonella typhimurium
Anaerobiosis
Phospholipase
Salmonella
Microbiology
Enzyme
Virulence
Bacteria
Hydrolases
Esterases
Enterobacteriaceae
Online AccessGet full text

Cover

More Information
Summary:Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) can initiate entry into non-phagocytic epithelial cells by triggering certain signal transduction pathways, thereby allowing the pathogen to invade and establish a niche within host cells. Anaerobiosis has been shown to be an important inducer of the invasion process of S. Typhimurium. However, the effect of anaerobiosis on modulation of cell signalling cascades by S. Typhimurium is not known. In the present study, the phospholipase C gamma signalling cascade was investigated in mice enterocytes, following interaction with S. Typhimurium grown under aerobic and anaerobic growth conditions. Significant increases in enterocyte intracellular calcium and inositol 1,4,5-triphosphate levels were observed on interaction with S. Typhimurium grown anaerobically compared with S. Typhimurium grown aerobically. An increased membrane/cytosolic ratio of protein kinase C was also seen with anaerobic S. Typhimurium in enterocytes compared with aerobic S. Typhimurium. These data suggest that anaerobically grown organisms are more efficient in initiating cell-signalling events than are aerobically grown bacteria. These enhanced cell signals may contribute to the increased virulence of S. Typhimurium grown anaerobically.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0022-2615
1473-5644
DOI:10.1099/jmm.0.05186-0