RNase H-dependent DNA thresholder modulated by cancer marker concentration
Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations. The mRNA cleavage is activated by 2.6, 7.5 or 39.5 nM of biomarker depending on the construct design. The constructs can be used to differentiate cancer from normal cells by the lev...
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Published in | Chemical communications (Cambridge, England) Vol. 6; no. 33; pp. 4427 - 443 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Royal Society of Chemistry
18.04.2024
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Subjects | |
Online Access | Get full text |
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Summary: | Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations. The mRNA cleavage is activated by 2.6, 7.5 or 39.5 nM of biomarker depending on the construct design. The constructs can be used to differentiate cancer from normal cells by the level of oncogene expression followed by silencing of a targeted gene.
Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations. |
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Bibliography: | Electronic supplementary information (ESI) available. See DOI https://doi.org/10.1039/d4cc00495g ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1359-7345 1364-548X |
DOI: | 10.1039/d4cc00495g |