RNase H-dependent DNA thresholder modulated by cancer marker concentration

Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations. The mRNA cleavage is activated by 2.6, 7.5 or 39.5 nM of biomarker depending on the construct design. The constructs can be used to differentiate cancer from normal cells by the lev...

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Published inChemical communications (Cambridge, England) Vol. 6; no. 33; pp. 4427 - 443
Main Authors Nour, Moustapha A. Y, Drozd, Valeriia S, Lemeshko, Evgenii A, Tafran, Lilia, Salimova, Adeliia A, Kulikova, Alexandra V, Eldeeb, Ahmed A
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 18.04.2024
Subjects
Biomarkers
Cancer
DNA
Endoribonucleases
Humans
Neoplasms
Ribonuclease H - metabolism
Ribonuclease, Pancreatic
Ribonucleases
RNA, Messenger - metabolism
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Summary:Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations. The mRNA cleavage is activated by 2.6, 7.5 or 39.5 nM of biomarker depending on the construct design. The constructs can be used to differentiate cancer from normal cells by the level of oncogene expression followed by silencing of a targeted gene. Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations.
Bibliography:Electronic supplementary information (ESI) available. See DOI
https://doi.org/10.1039/d4cc00495g
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/d4cc00495g