Organic small molecule for detection and photodegradation of mitochondrial DNA mutations

• Published in: Journal of materials chemistry. B, Materials for biology and medicine Vol. 7; no. 39; pp. 5947 - 5955
• Main Authors: Su, Chien-Hui, Chen, Jyun-Wei, Chen, Li-Da, Chang, Jui-Chih, Liu, Chin-San, Chang, Cheng-Chung, Wang, Gou-Jen
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 09.10.2019
• Subjects: Electron transfer; Energy transfer; Fluorescence; Fluorescence resonance energy transfer; Irradiation; Mitochondria; Mitochondrial DNA; Mutation; Optical properties; Photodegradation; Radiation; Reactive oxygen species
• ISSN: 2050-750X; 2050-7518
• DOI: 10.1039/c9tb01358j

A detection and degradation platform was developed to optically quantify the 6-enolate, 8-keto-dG, an important tautomer of mitochondrial mutated DNA 8-oxo-dG. We first found that 6-enolate, 8-keto-dG offers particular fluorescence emission under the conditions between pH ∼ 7 and ∼11. Thus, a mitochondria-targeting photosensitizer NV-12P was prepared to offer simultaneously photoinduced electron transfer and fluorescence resonance energy transfer (FRET) with 6-enolate, 8-keto-dG. Furthermore, NV-12P can also generate a reactive oxygen species to degrade 6-enolate, 8-keto-dG under irradiation conditions. This is the first publication about optical characterization, concentration detection and photodegradation of 6-enolate, 8-keto-dG, either in biological or in vitro applications. Mitochondrial localization photosensitizer NV-12P may recognize 8-oxo-dG with PET and FRET mechanism. Furthermore, NV-12P can generate a reactive oxygen species to degrade 8-oxo-dG using a similar mechanism of methyl blue.