Multivariate spectroscopic determination of the lamivudine-zidovudine association

The multivariate spectroscopic determination of lamivudine-zidovudine associations was carried out by partial least square regression (PLS). This model was developed from 20 synthetic mixtures using mean-centered spectral data acquired from 190 to 350 nm and with 3 latent variables. External validat...

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Bibliographic Details
Published inJournal of the Brazilian Chemical Society Vol. 22; no. 2; pp. 337 - 343
Main Authors Cordeiro, Gilcélia A, Nagata, Noemi, Messerschmidt, Iara, Peralta-Zamora, Patricio, Rodrigues, Letícia N. C
Format Journal Article
LanguageEnglish
Published Sociedade Brasileira de Química 01.02.2011
Subjects
CHEMISTRY, MULTIDISCIPLINARY
electronic spectroscopy
multivariate calibration
zidovudine
lamivudine
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Summary:The multivariate spectroscopic determination of lamivudine-zidovudine associations was carried out by partial least square regression (PLS). This model was developed from 20 synthetic mixtures using mean-centered spectral data acquired from 190 to 350 nm and with 3 latent variables. External validation was performed with 6 synthetic mixtures providing prediction errors close to 1%. Moreover, the analysis of commercial drugs showed good results with prediction errors lower than 10%. The multivariate methodology was validated according to International Conference on Harmonization (ICH) criteria, demonstrating precision, accuracy and robustness within legal requirements.
ISSN:0103-5053
1678-4790
DOI:10.1590/S0103-50532011000200020