Chemiluminescence determination of mezlocillin by the luminol-potassium periodate system

A new, simple and sensitive method has been proposed for the determination of mezlocillin. It is based on the enhancement of the chemiluminescence (CL) emission generated from the oxidation of luminol in alkaline medium by postassium periodate. The optimization of the experimental and instrumental v...

Full description

Saved in:
Bibliographic Details
Published inJournal of the Brazilian Chemical Society Vol. 19; no. 6; pp. 1180 - 1185
Main Authors Shi, Wen Bing, Yang, Ji Dong
Format Journal Article
LanguageEnglish
Published Sociedade Brasileira de Química 2008
Subjects
CHEMISTRY, MULTIDISCIPLINARY
chemiluminescence
flow injection
mezlocillin
Online AccessGet full text

Cover

More Information
Summary:A new, simple and sensitive method has been proposed for the determination of mezlocillin. It is based on the enhancement of the chemiluminescence (CL) emission generated from the oxidation of luminol in alkaline medium by postassium periodate. The optimization of the experimental and instrumental variables affecting the CL enhancement effect has been carried out using flow-injection system. In the optimum conditions, the method is efficient to determine mezlocillin in the linear range of 0.01-30×10-6 g mL-1 with a detection limit (3σ) of 3.0×10-9 g mL-1 and the relative standard deviation (RSD) is 1.0% for 1.0×10-6 g mL-1 mezlocillin (n=11). It has been successfully applied to the mezlocillin determination in commercial preparations, synthetic samples and biological fluids formulations.
ISSN:0103-5053
1678-4790
DOI:10.1590/S0103-50532008000600019