Dynamic selection of high-affinity aptamers using a magnetically activated continuous deflection microfluidic chip

• Published in: Chemical communications (Cambridge, England) Vol. 6; no. 2; pp. 2772 - 2775
• Main Authors: Yang, Ke-Zhu, Wang, Meng, Gao, Ming-Yue, Wang, Yong-Tao, Zhang, Zhi-Ling
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 05.03.2024
• Subjects: Affinity; Continuous flow; Deflection
• ISSN: 1359-7345; 1364-548X
• DOI: 10.1039/d4cc00229f

To accelerate the discovery of high-affinity aptamers, a magnetically activated continuous deflection (MACD) chip was designed. The MACD chip could achieve dynamic selection in a continuous flow, which meant that the binding and separation were carried out consecutively. Dynamic selection could make selection efficient. Low-affinity sequences could be eluted in time and high-affinity sequences could be enriched via dynamic selection. The stringency of the conditions could be further increased by lowering the target concentration in the dynamic selection. Finally, a C.al3 aptamer with high-affinity and high-specificity for Candida albicans ( C. albicans ) was obtained through six rounds of selection. Its dissociation constant ( K d ) was 7.9 nM. This demonstrated that dynamic selection using a MACD chip was an effective method for high-affinity aptamer selection. A MACD chip was designed for the discovery of Candida albicans aptamers via dynamic selection.