A molecular chemodosimeter to probe "closed shell" ions in kidney cells

• Published in: Organic & biomolecular chemistry Vol. 21; no. 47; pp. 9379 - 9391
• Main Authors: Assel, Amine, Stanley, Meagan M, Mia, Rashid, Boulila, Besma, Cragg, Peter J, Owolabie, Iyanuoluwani, Hetrick, Meredith, Flynt, Alex, Wallace, Karl J, Ben Jannet, Hichem
• Format: Journal Article
• Language: English
• Published: CAMBRIDGE Royal Soc Chemistry 06.12.2023; Royal Society of Chemistry
• Subjects: Aqueous solutions; Carbon dioxide; Cations; Chemical synthesis; Chemistry; Chemistry, Organic; Cobalt; Confocal microscopy; Copper; Coumarin; Excimers; Fluorescence; Fluorescent Dyes; HEK293 Cells; HeLa Cells; Humans; Ions; Kidneys; Mercury; Metal ions; Metals; Physical Sciences; Quinidine; Science & Technology; Zinc; QUINOLINE; METAL-ION; FLUORESCENT SENSOR; CU(II); SENSITIVITY; COPPER; ENAMINONE; ZINC
• ISSN: 1477-0520; 1477-0539
• DOI: 10.1039/d3ob01408h

Two quinidine-functionalized coumarin molecular probes have been synthesized and have been found to bind metal cations (Cd 2+ , Co 2+ , Cu 2+ , Fe 2+ , Hg 2+ , Ni 2+ , and Zn 2+ ) with high affinity in organic-aqueous media (DMSO-HEPES). The chemodosimeters coordinate with the Zn 2+ ions in a two-to-one ratio (molecular probe : Zn 2+ ) with a log  β of 10.0 M −2 . Upon the addition of the closed-shell metal ions studied, a fluorescence turn-on via an excimer formation is seen at 542 nm due to the quinaldine moiety adopting a syn arrangement when coordinated to the metal Zn 2+ ions. Confocal microscopy monitored free Zn 2+ ions in the Human Embryonic Kidney cell line HEK293 by coordinating with the chemodosimter. Two chemodosimeters have been synthesized to detect closed-shell metal ions (Zn 2+ and Cd 2+ ) in HEK 293 cells.