Two highly sensitive and selective coumarin-based fluorometric probes for the detection of ClO − and cell imaging
Two coumarin-based compounds, 2-acetyl-3 H -benzo[ f ]chromen-3-one ( BCO ) and (1 E )-1-(1-(3-oxo-3 H -benzo[ f ]-chromen-2-yl)ethylidene)thiosemicarbazide ( BETC ), were designed as excellent fluorescent probes for the detection of ClO − in CH 3 OH : H 2 O (v/v = 1 : 1). The oxidizing reaction bet...
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Published in | Analytical methods Vol. 11; no. 14; pp. 1916 - 1922 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Cambridge
Royal Society of Chemistry
14.04.2019
|
Subjects | |
Online Access | Get full text |
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Summary: | Two coumarin-based compounds, 2-acetyl-3
H
-benzo[
f
]chromen-3-one (
BCO
) and (1
E
)-1-(1-(3-oxo-3
H
-benzo[
f
]-chromen-2-yl)ethylidene)thiosemicarbazide (
BETC
), were designed as excellent fluorescent probes for the detection of ClO
−
in CH
3
OH : H
2
O (v/v = 1 : 1). The oxidizing reaction between the
BCO
&
BETC
probes and ClO
−
induced an obvious fluorescence enhancement together with a color transformation from colorless to blue. The probes showed preferential selectivity towards ClO
−
over various anions and metal ions. The detection limits of
BCO
and
BETC
were 154 nM and 32 nM, respectively. Interestingly, the tested reactive species, which include ˙OH,
1
O
2
, and H
2
O
2
do not interfere with the tests of ClO
−
. Furthermore, cell imaging using HepG2 cells revealed that
BCO
&
BETC
could be applied as effective fluorescent probes for the detection of ClO
−
in HepG2 cells. |
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ISSN: | 1759-9660 1759-9679 |
DOI: | 10.1039/C9AY00105K |