Two highly sensitive and selective coumarin-based fluorometric probes for the detection of ClO − and cell imaging

Two coumarin-based compounds, 2-acetyl-3 H -benzo[ f ]chromen-3-one ( BCO ) and (1 E )-1-(1-(3-oxo-3 H -benzo[ f ]-chromen-2-yl)ethylidene)thiosemicarbazide ( BETC ), were designed as excellent fluorescent probes for the detection of ClO − in CH 3 OH : H 2 O (v/v = 1 : 1). The oxidizing reaction bet...

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Bibliographic Details
Published inAnalytical methods Vol. 11; no. 14; pp. 1916 - 1922
Main Authors Jin, Lei, Tan, Xiaoxue, Zhao, Cong, Wang, Qingming
Format Journal Article
LanguageEnglish
Published Cambridge Royal Society of Chemistry 14.04.2019
Subjects
Anions
Coumarin
Detection limits
Fluorescence
Fluorescent indicators
Hydrogen peroxide
Imaging
Metal ions
Oxidation
Probes
Selectivity
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Summary:Two coumarin-based compounds, 2-acetyl-3 H -benzo[ f ]chromen-3-one ( BCO ) and (1 E )-1-(1-(3-oxo-3 H -benzo[ f ]-chromen-2-yl)ethylidene)thiosemicarbazide ( BETC ), were designed as excellent fluorescent probes for the detection of ClO − in CH 3 OH : H 2 O (v/v = 1 : 1). The oxidizing reaction between the BCO & BETC probes and ClO − induced an obvious fluorescence enhancement together with a color transformation from colorless to blue. The probes showed preferential selectivity towards ClO − over various anions and metal ions. The detection limits of BCO and BETC were 154 nM and 32 nM, respectively. Interestingly, the tested reactive species, which include ˙OH, 1 O 2 , and H 2 O 2 do not interfere with the tests of ClO − . Furthermore, cell imaging using HepG2 cells revealed that BCO & BETC could be applied as effective fluorescent probes for the detection of ClO − in HepG2 cells.
ISSN:1759-9660
1759-9679
DOI:10.1039/C9AY00105K