1,25-dihydroxyvitamin D3 regulates LPS-induced cytokine production and reduces mortality in rats

• Published in: World journal of gastroenterology : WJG Vol. 14; no. 24; pp. 3897 - 3902
• Main Authors: Qi, Xiao-Ping, Li, Pei, Li, Gang, Sun, Zhen, Li, Jie-Shou
• Format: Journal Article
• Language: English
• Published: School of Medicine,Nanjing University,Department of General Surgery,Jinling Hospital,305 Zhongshandong Road,Nanjing 210002,Jiangsu Province,China 2008; The WJG Press and Baishideng
• Subjects: Rapid Communication; 免疫控制; 内毒素; 死亡率; 细胞因子; Immunoregulation; Mortality; Endotoxin,Cytokine,1,25-dihydroxy-vitamin-D3
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.14.3897

AIM： To study the immunoregulatory effect of 1,25-dihydroxyvitamin-D3 Von dominant Thl response in rats. METHODS： Sixty adult Lewis rats were randomized into three groups. Rats in group 1 （n=25） were treated with 1,25-（OH）2D3 first and then challenged with LPS, rats in group 2 （n=25） were treated with vehicle first and then challenged with LPS. Ten animals in groups 1 and 2 were preserved for mortality observation. The remaining animals were injected （i.p） with endotoxin, 24 h after the last administration of 1,25-（OH）2D3 and vehicle. Rats in group 3 （n=10） were treated with 1,25-（OH）2D3 only. Serum IL-12, IFN-y, IL-2 and IL-4 levels were measured and target gene of 1,25-（OH）2D3 on Th cells was studied after 6 h. Gene abundance was verified by real-time quantitative PCR. RESULTS： No death occurred in rats pretreated with 1,25-（OH）2D3 after LPS injection. Death occurred 9 h after LPS injection in rats pretreated with the vehicle, and the number of deaths was 5 within 24 h, with a mortality rate of 50%. There was no change in the number of deaths within 96 h. Six hours after endotoxin stimulation, serum IL-12 and IFN-y levels decreased significantly in rats pretreated with 1,25-（OH）2D3 as compared with those in rats pretreated with the vehicle. The serum content of these two cytokines was very low in rats not challenged by endotoxin, and there was a significant difference as compared with the previous two groups. CONCLUSION： 1,25-（OH）2D3 attenuates injuryinduced by the lethal dose of 1PS, regulates Thl and Th2 cells at the transcription level, and dominantly responds to cytokine production in rats.