THUMPD3-AS1 Is Correlated With Non-Small Cell Lung Cancer And Regulates Self-Renewal Through miR-543 And ONECUT2

• Published in: OncoTargets and therapy Vol. 12; pp. 9849 - 9860
• Main Authors: Hu, Jia, Chen, Youfang, Li, Xiaodong, Miao, Huikai, Li, Rongzhen, Chen, Dongni, Wen, Zhesheng
• Format: Journal Article
• Language: English
• Published: New Zealand Dove 2019
• Subjects: Original Research; non-small cell lung cancer; miR-543; THUMPD3-AS1; ONECUT2; self-renewal
• ISSN: 1178-6930; 1178-6930
• DOI: 10.2147/OTT.S227995

Of all malignancies, lung cancer is the leading cause of death, and non-small cell lung cancer (NSCLC) accounts for 80-85% of all lung cancers. In this study, the long non-coding RNA (lncRNA) THUMPD3-AS1 was observed to be highly expressed in NSCLC and correlated with TNM stages and relapse, suggesting that THUMPD3-AS1 is involved in the regulation of NSCLC. The aim of this study was to investigate the regulatory function and mechanism of THUMPD3-AS1 in NSCLC cells by cellular function and molecular biology experiments. Overexpression and knockdown analysis revealed that THUMPD3-AS1 promoted tumor progression by increasing cell proliferation and self-renewal of NSCLC cells. Moreover, THUMPD3-AS1 may act as an endogenous sponge of microRNA-543 (miR-543) which can regulate the target gene ONECUT2 in NSCLC cells. Our study indicated that THUMPD3-AS1 regulated NSCLC cell self-renewal by regulating the expression of miR-543 and ONECUT2, and THUMPD3-AS1 can potentially act as a biomarker or therapeutic target in NSCLC.