MicroRNA-183-5p Inhibits Aggressiveness of Cervical Cancer Cells by Targeting Integrin Subunit Beta 1 (ITGB1)

• Published in: Medical science monitor Vol. 24; pp. 7137 - 7145
• Main Authors: Zhang, Wei, Zhang, Mingkai, Liu, Lantao, Jin, Dan, Wang, Pengyu, Hu, Jing
• Format: Journal Article
• Language: English
• Published: United States International Scientific Literature, Inc 07.10.2018
• Subjects: 3' Untranslated Regions; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Female; Humans; Integrin beta1 - genetics; Integrin beta1 - metabolism; Lab/In Vitro Research; MicroRNAs - genetics; MicroRNAs - metabolism; Neoplasm Invasiveness - genetics; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - metabolism; Uterine Cervical Neoplasms - pathology
• ISSN: 1643-3750; 1234-1010; 1643-3750
• DOI: 10.12659/MSM.910295

BACKGROUND Accumulating studies demonstrate that microRNAs play crucial roles in multiple processes of cancer progression. Lower levels of miR-183 have been observed in diverse types of tumors but the mechanism and precise function of miR-183-5p in cervical cancer have largely not been investigated. MATERIAL AND METHODS The level of miR-183-5p in different cervical cancer cell lines and clinical tissues was detected qRT-PCR assays. Transwell and wound-healing migration assays were conducted to assess the functional roles of miR-183-5p in over-expressing cervical cancer cells in vitro. Rescue assays were carried out to confirm the contribution of integrin subunit Beta 1 (ITGB1) to the aggressiveness of cancer cells regulated by miR-183-5p. RESULTS miR-183-5p was reduced in clinical tissues of cervical cancer and cell lines when compared to the normal subjects and normal cervical epithelial cell line, respectively. In addition, over-expression of miR-183-5p markedly inhibited migration and invasion in cervical cancer cells, and increased aggressiveness was observed in miR-183-5p inhibitor transfected cells. Furthermore, the luciferase reporter assays revealed that ITGB1 was the gene directly regulated by miR-183-5p. Notably, a negative association between the ITGB1 and miR-183-5p was found, and the gene expressions of ITGB1 was mediated by miR-183-5p in cervical cancer cells. CONCLUSIONS miR-183-5p serves as a latent anti-oncogene by targeting the metastasis-promoter gene, ITGB1.