A novel Streptomyces spp. integration vector derived from the S. venezuelaephage, SV1

Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 int...

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Bibliographic Details
Published inBMC biotechnology Vol. 14; no. 1
Main Authors Fayed, Bahgat, Younger, Ellen, Taylor, Gabrielle, Smith, Margaret C M
Format Journal Article
LanguageEnglish
Published BioMed Central Ltd 30.05.2014
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Summary:Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1. pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.
ISSN:1472-6750
1472-6750
DOI:10.1186/1472-6750-14-51