Diplocloster agilis gen. nov., sp. nov. and Diplocloster modestus sp. nov., two novel anaerobic fermentative members of Lachnospiraceae isolated from human faeces

• Published in: International journal of systematic and evolutionary microbiology Vol. 72; no. 2
• Main Authors: Chaplin, Andrei V, Shcherbakova, Viktoria A, Pikina, Alla P, Sokolova, Sofia R, Korzhanova, Margarita, Belova, Vera A, Korostin, Dmitriy O, Rebrikov, Denis V, Kardonsky, Dmitry A, Urban, Anatoly S, Zakharzhevskaya, Natalia B, Suzina, Natalia E, Podoprigora, Irina V, Das, Milana S, Kholopova, Daria O, Efimov, Boris A
• Format: Journal Article
• Language: English
• Published: England 01.02.2022
• Subjects: Anaerobiosis; Bacterial Typing Techniques; Base Composition; Child; DNA, Bacterial - genetics; Fatty Acids - chemistry; Feces - microbiology; Firmicutes - classification; Firmicutes - isolation & purification; Humans; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Diplocloster; Lachnospiraceae; human gut microbiota; novel species
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.005222

Three novel strains of Gram-stain-negative, obligately anaerobic, spore-forming straight or slightly curved rods with pointed ends occurring singly or in pairs were isolated from the faeces of healthy human children. The strains were characterized by mesophilic fermentative metabolism and production of acetate, ethanol and H as the end metabolic products. Strains ASD3451 and ASD5720 were motile, fermented lactose and raffinose, and weakly fermented maltose. Strain ASD4241 was non-motile and did not ferment the carbohydrates listed above but fermented starch. Strains ASD3451 and ASD5720 shared average nucleotide identity higher than 98.5 % with each other, while ASD4241 had only 88.5-89 % identity to them. Based on phylogenetic and chemotaxonomic analyses, we propose gen. nov., sp. nov. (ASD5720 =JCM 34353 =VKM B-3497 ) and sp. nov. (ASD4241 =JCM 34351 =VKM B-3498 ) within the family .