Fluorescence diffuse tomography technique with autofluorescence removal based on dispersion of biotissue optical properties

• Published in: Laser physics letters Vol. 10; no. 7; pp. 75601 - 75606
• Main Authors: Kleshnin, M S, Turchin, I V
• Format: Journal Article
• Language: English
• Published: IOP Publishing 01.07.2013
• ISSN: 1612-2011; 1612-202X
• DOI: 10.1088/1612-2011/10/7/075601

The dispersion of biotissue optical properties results in distortion of the spectrum of radiation during propagation through biotissues. This phenomenon can be used for solving the inverse problem in fluorescence diffuse tomography. We have developed the spectrally resolved fluorescence diffuse tomography (SFDT) technique, which allows reconstruction of the spatial distribution of the fluorophore in biotissue even in the presence of unknown autofluorescence. The experimental setup combining epi-illumination and trans-illumination imaging geometries with spectral resolution has been created. Experimental studies on tissue phantoms and small animals in vivo with the proposed SFDT technique have been conducted. The obtained results have shown high accuracy of the inverse problem solution for spatial distribution of fluorophore concentration.