Peribacillus faecalis sp. nov., a moderately halophilic bacterium isolated from the faeces of a cow

• Published in: International journal of systematic and evolutionary microbiology Vol. 71; no. 3
• Main Authors: Jiang, Lingmin, Jung, Won Yong, Li, Zhun, Lee, Mi-Kyung, Park, Seung-Hwan, Kang, Se Won, Lee, Jung-Sook, Jung, Hyunjung, Hur, Tai-Young, Kim, Hyeun Bum, Kim, Jae-Kyung, Lee, Ju-Hoon, Lee, Ju Huck, Lee, Jiyoung
• Format: Journal Article
• Language: English
• Published: England 01.01.2021
• Subjects: phylogeny; Peribacillus faecalis sp. nov; novel species; polyphasic taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.004721

A Gram-stain-positive, facultatively anaerobic, endospore-forming, rod-shaped strain, AGMB 02131 , which grew at 20-40 °C (optimum 30 °C), pH 3.0-11.0 (optimum pH 4.0) and in the presence of 0-18 % (w/v) NaCl (optimum 10 %), was isolated from a cow faecal sample and identified as a novel strain using a polyphasic taxonomic approach. The phylogenetic analysis based on 16S rRNA gene sequences along with the whole genome (92 core gene sets) revealed that AGMB 02131 formed a group within the genus , and showed the highest sequence similarity with DSM 28131 (96.9 %), following by DSM 18926 (96.6 %). The genome of AGMB 02131 comprised 70 contigs, the chromosome length was 4 038 965 bp and it had a 38.5 % DNA G+C content. Digital DNA-DNA hybridization revealed that AGMB 02131 displayed 21.4 % genomic DNA relatedness with the most closely related strain, DSM 18926 . AGMB 02131 contains all of the conserved signature indels that are specific for members of the genus . The major cellular fatty acids (>10 %) of AGMB 02131 were C ω9 , C and C . The major polar lipids present were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. On the basis of the phenotypic, phylogenetic, genomic and chemotaxonomic features, AGMB 02131 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AGMB 02131 (=KCTC 43221 =CCTCC AB 2020077 ).